Figure 8.

Effects of inhibiting K2P channels on saltatory conduction at the NR of lumbar spinal ventral nerves. A, Summary data of AP CV determined in the absence (control) and presence of 200 μm RR (n = 7). B, AP success rates at NRs with distal stimulation at frequency of 1, 10, 100, 200, 500, and 1000 Hz (n = 7) determined in control and the presence of 200 μm RR (n = 7). Recording duration at each frequency was 20 s. C, Bar graphs represent summary data of FS₅₀ (n = 7) in control and the presence of 200 μm RR (n = 7). D, Summary data of AP CV determined in the absence (control) and presence of 5 mm Ba²⁺ (n = 7). E, AP success rates at NRs with distal stimulation at frequency of 1, 10, 100, 200, 500, and 1000 Hz (n = 5) determined in control and the presence of 5 mm Ba²⁺ (n = 5). Recording duration at each frequency was 20 s. F, Bar graphs represent summary data of FS₅₀ (n = 5) in control and the presence of 5 mm Ba²⁺ (n = 5). G, Summary data of AP CV determined in the absence (control) and presence of 1 μm spadin (n = 7). H, AP success rates at NRs with distal stimulation at frequency of 1, 10, 100, 200, 500, and 1000 Hz (n = 7) determined in control and in the presence of 1 μm spadin (n = 7). Recording duration at each frequency was 20 s. I, Bar graphs represent summary data of FS₅₀ (n = 7) in control and the presence of 1 μm spadin (n = 7). In all experiments, whole-cell current-clamp recordings were performed at NRs and APs were evoked by electrical stimulation from distal sites of nerve bundles. Data represent mean ± SEM. *p < 0.05; **p < 0.01; Student's t test. ns, p ≥ 0.05.