Figure 8.

TMEM59 deficiency attenuates microglial inflammation but has minimal effect on microglial density and morphology in mice. A–D, qRT-PCR analysis of Tnfα, Il-1β, Arg1, and Ym-1 mRNA levels in primary microglia from 59^fl/fl;Lyz2 mice and controls. n = 6. E, Confocal images showing microglial density (green, Iba1⁺) in cortex (Ctx) and hippocampal CA1, CA3, and DG regions of 59^fl/fl and 59^fl/fl;Lyz2 mice (P19–P22). Scale bar: 100 µm. F, Quantification of Iba1⁺ microglial cells in Ctx, CA1, CA3, and DG regions. n = 3 mice per group. G, Representative 3D reconstruction of microglial morphology in Ctx and CA1 regions of 59^fl/fl and 59^fl/fl;Lyz2 mice (P19–P22). Scale bar: 15 µm. H, I, Quantification of microglial morphologic parameters, including path length (H) and branch numbers (I) in Ctx and CA1 regions. n = 3 mice per group, average of 7–9 cells from each mouse. Data represent mean ± SEM; **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: not significant, unpaired t test.