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. 2022 Jun;30(2):100–104. doi: 10.5455/aim.2022.30.100-104

Table 2. Characteristics of the samples after preparation using the 3 tested methods.

Variable Mini-gradient
Mean ± SD
Single 90-layer
Mean ± SD
Single 45-layer
Mean ± SD
Concentration (106 cells/mL) 10.8 ± 4.8a 12.0 ± 5.5a 19.2 ± 8.0b
Progressive motility (PR, %) 61.3 ± 21.8a 61.7 ± 18.5a 47.8 ± 15.8c
Total motility
(PR + NP, %)
72.3 ± 15.4a 72.8± 15.1a 58.7 ± 13.0b
Vitality (%) 81.4 ± 11.6a 80.1 ± 12.0a 67.6 ± 11.5b