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. Author manuscript; available in PMC: 2022 Jun 25.
Published in final edited form as: Science. 2022 Apr 1;376(6588):eabl4178. doi: 10.1126/science.abl4178

Fig. 4. Inner kinetochore associates with recently expanded αSat HORs.

Fig. 4.

(A) Active αSat HOR array on chr12 (coordinates at top). Track 1, CENP-A NChIP-seq marker-assisted mapping coverage. Track 2, reference-free region-specific marker enrichment (black indicates no markers in bin) (42). Track 3, percent of CpG sites methylated. Tracks 4 and 5, HOR-haps (k = 5 or 2 clusters, respectively). Track 6, number of HOR units (out of 10 per bin) that have at least one identical copy in the array. (Bottom) Self-alignment dotplot (exact-match word size 2000), with arrows pointing to a zone of recent duplication. (Inset) Smaller dotplot of the entire array (word size 500, allowing for detection of older duplications), with positions of two large macro-repeats indicated with blue lines. (B) As in (A) but for chr4. (Inset) Highlighting of a secondary CENP-A enrichment site and minor CDR on the other side of the interrupting HSat1A array. (C) As in (A) but for chr6, with CENP-A enrichment over an older HOR-hap region. (D) Rooted HOR-hap consensus phylogenetic trees as in Fig. 3F, with CENP-A–enriched region(s) indicated with arrows.