Figure 1.
Characterization of hUC-MSCs. (a) Trilineage differentiation of hUC-MSCs. Adipocyte differentiation capacity of hUC-MSCs was confirmed by Oil Red O staining. Osteogenic differentiation potential of hUC-MSCs was verified by Alizarin red staining. Chondrogenic differentiation ability of hUC-MSCs was proved by Alcian blue staining. Scale bar = 1000 μm. (b) Flow cytometry analysis of mesenchymal markers (CD34-, CD45-, HLA-DR-, CD29+, CD73+, CD90+, CD105+, and CD44+) and the corresponding isotype controls (mouse IgG1 Kappa, rat IgG2b Kappa, and mouse IgG2b Kappa).