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. 2022 Jun 25;12:10806. doi: 10.1038/s41598-022-14995-0

Table 1.

Selected mutations for SSIIIa homoeologues.

Gene Line Codon Protein coordinate and predicted effect Chrom Exon Protein domain
SSIIIa-A C2074 Cag → Tag Q 531 (stop codon) 1A 3 Variable repeat region
SSIIIa-B C0905 Caa → Taa Q 1306 (stop codon) 1B 10 Catalytic domain
SSIIIa-D C0291 Cga → Tga R 435 (stop codon) 1D 3 Variable repeat region

Line is the mutant number in the TILLING population (e.g., C2074 = Cadenza 2074). Codon is the sequence of the control (left) and mutant (right) codons; the uppercase letter indicates which base was mutated. Protein coordinate includes the affected amino acid reside and the position based on deduced amino acid sequences from SSIIIa genomic DNA in the Cadenza cultivar. Chrom is the homoeologous chromosome. Protein domain is based on the defined proteins domains described in Li et al.8