Table 1.
Gene | Line | Codon | Protein coordinate and predicted effect | Chrom | Exon | Protein domain |
---|---|---|---|---|---|---|
SSIIIa-A | C2074 | Cag → Tag | Q 531 (stop codon) | 1A | 3 | Variable repeat region |
SSIIIa-B | C0905 | Caa → Taa | Q 1306 (stop codon) | 1B | 10 | Catalytic domain |
SSIIIa-D | C0291 | Cga → Tga | R 435 (stop codon) | 1D | 3 | Variable repeat region |
Line is the mutant number in the TILLING population (e.g., C2074 = Cadenza 2074). Codon is the sequence of the control (left) and mutant (right) codons; the uppercase letter indicates which base was mutated. Protein coordinate includes the affected amino acid reside and the position based on deduced amino acid sequences from SSIIIa genomic DNA in the Cadenza cultivar. Chrom is the homoeologous chromosome. Protein domain is based on the defined proteins domains described in Li et al.8