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. 2022 Jun 25;13:3652. doi: 10.1038/s41467-022-31376-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a UMAP overlaying MP2 samples and their distinct clusters. b Bar graph displaying distribution of cells per MP2 culture across clusters; MP2-A in red, MP2-B in green and MP2-C in blue. c Bubble plot showing enrichment of pathways in MP2 cultures compared to each other with normalized enrichment score (NES) on the x-axis. Size of the bubble represents false discovery rate (FDR); red indicates upregulation, blue indicates downregulation of the pathway. d inferCNV heatmaps derived from scRNAseq data of all three MP2 samples, using HPNE as an analytical reference.