Fig. 2. RNASE4 protein levels in the plasma tissues of prostate cancer patients are correlated with poor prognosis and high risk of metastasis.

a–d Correlation of plasma RNASE4 protein levels with tumor surgical T-stage (a), clinical stage (b), biopsy grade (c), and surgical Gleason (d). RNASE4 levels in the plasma were determined by ELISA. e–i Correlation of tissue RNASE4 levels with tumor histological grade (e), Gleason score (f), tumor stage (g), distant metastasis (h), and lymph node metastasis (i). Tissue RNASE4 levels were determined by semi-quantitative IHC. Each dot represents an individual sample. Lines represent the median values and the interquartile ranges. Statistical analyses were done by one-way ANOVA (a, b) and two-tailed Student’s t test (c–i).