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. 2022 Jun 25;5:625. doi: 10.1038/s42003-022-03597-1

Fig. 6. RNASE4 mAb inhibits prostate cancer cell proliferation and RNASE4-induced angiogenesis.

Fig. 6

a Effect of RNASE4 mAb on cell proliferation. DU145, PC-3, and LNCaP cells were cultured in the presence of 2% FBS. RNASE4 mAb or isotype control IgG2bκ were added at the concentrations indicated. Cell numbers were determined by a Coulter counter. b Effect of RNASE4 mAb on cell cycle distribution of DU145 cells. Cells were cultured in the 2% FBS and RNASE4 mAb (30 µg/ml) or isotype control IgG2bκ (30 µg/ml) for 3 days. Cell cycle status was determined by flow cytometry. c Effect of RNASE4 mAb on endothelail cell tube formation. HUVEC were cultured on Matrigel-coated wells and incubated in the absence or presence of 1 µg/m of RNASE4 with 30 µg/ml of RNASE4 mAb or isotype control IgG2bκ for 4 h. Top panels, images of endothelial cell tubular structure of a representative experiment in duplicate of 3 independent repeats. Scale bar = 50 µm. Bottom panels, tube length and number of loops counted from 5 randomly selected areas. Data shown are means ± SEM. *p ≤ 0.05, **p ≤ 0.01 and ***p ≤ 0.001, by two-tailed Student’s t test.