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. 2022 Jun 25;13:3646. doi: 10.1038/s41467-022-31220-8

Fig. 4. WASP deficiency results in altered RNA splicing in iMPs and B-cell lines.

Fig. 4

a Altered alternative splicing events (AASEs) in KO-iMPs (three clones). A3SS alternative 3’ splicing site, A5SS alternative 5’ splicing site, MXE multi-exon skipping, RI intron retention, SE exon skipping. WT iMP biological replicate n = 2, KO-iMP biological replicate n = 3. b AASEs in 3 WASP mutant B-cell lines. Biological replicate n = 2. c GO enrichment of AASEs in KO-iMPs. WT-iMP biological replicate n = 2, KO-iMP biological replicate n = 3. d GO enrichment of common AASEs in three WASP mutant B-cell lines. Biological replicate n = 2. Hypergeometric testing P values are corrected for multiple testing using a false discovery rate (FDR). e AASEs in both upregulated genes and downregulated genes in KO-iMPs. up upregulated genes, dw downregulated genes. f Splicing-factor motif analysis of altered SE, RI, and MXE events in KO-iMPs. g Splicing-factor motif analysis of the downstream exon of altered SE in WASnull B cells.