Table 2.
Precise genotype used for each figure panel.
| Figure | Genotype | Clone induction | Dev. time |
|---|---|---|---|
| 1b | E-cad-GFP(KI); pnr-Gal4 | – | 16 h APF |
| 1c, d | hs-flp22; ubi-E-cad-GFP UAS-RFP/+; act<y < Gal4/UAS-hid-dsRNA (quantification outside clones) | – | 16 h APF |
| 1e, f, i–k; S1 a, c–g | hs-flp22; sqh-Sqh-GFP; sqh-Sqh-GFP | – | 20 h APF |
| S1b | hsFLP; sqh-Sqh-GFP, FRT40A/ FRT40A, sqh-Sqh-RFP | 48 h ACI, 12 min hs | 16 h APF |
| 1g, h, S1h, i | hs-flp; act<y < gal4, UAS-mcd8RFP/Cyo; sqh-GFP-utABD/TM6b | – | 16 h APF |
| S1j–l | w; E-cad-tdTomato (KI)/+; +/ubi-mE-AnilRBDGFP | – | 16 h APF |
| S1m, n | hs-flp22; sqh-sqh::GFP/Cyo; act<cd2 < gal4, UAS-nlsRFP/UAS-hid-dsRNA | 48 h ACI, 12 min hs | 20 h APF |
| 2i | w; E-cad-GFP(KI) | – | |
| 2j | w; E-cad-tdTomato(KI)/+;pnr-Gal4/UAS-EB1-GFP | – | |
| S2a top | hs-flp22; sqh-Sqh::GFP; sqh-Sqh::GFP | – | 20 h APF |
| S2a bottom | w; E-cad-GFP(KI) | – | 20 h AFP |
| S2b, c | w; E-cad::3xmKate(KI); pnr-Gal4/UAS-ROK-dsRNA | – | 16 h APF |
| S2d, e | w; UAS-optoDroncCRY2-GFP/ E-cad-tdTomato; MKRS/act<y < Gal4 UAS-GFP | 48 h ACI, 12 min hs | 16 h APF |
| S2f | ubi-Talin::GFP/FM7 | – | 16 h APF |
| 3a, c | w; E-cad-tdTomato(KI) | – | 16 h APF |
| 3d–i | w; E-cad-tdTomato(KI)/cyo; Jupiter-GFP/TM6B | – | 16 h APF |
| S3a–g & l–n | w; E-cad-tdTomato(KI)/+; pnr-Gal4/UAS-EB1-GFP | – | 16 h APF |
| S3h | w; E-cad-GFP(KI)/+; pnr-Gal4/UAS-alpha-Tub-mCherry-human | – | 16 h APF |
| S3i | w; E-cad::3xmKate (KI)/shot(l)-GFP (TRAP) | – | 16 h APF |
| S3j | w;ubi-p63-Patronin.A.GFP/Cyo | – | 16 h APF |
| S3k | w; E-cad-tdTomato(KI)/ubi-Sas4-GFP | – | 16 h APF |
| S4a–c | w; E-cad-GFP(KI)/UAS-α-tubulin-mCherry; pnr-Gal4/+ | – | 16 h APF |
| S4d–e | hs-flp22; sqh-sqhmKatex3/+; act<y < Gal4, UAS-BFP/UAS-EB1-GFP | 48 h ACI, 12 min hs | 16 h APF |
| S4f, g | w; UAS-optoDroncCRY2-GFP/ E-cad-tdTomato(KI); MKRS/act<y < Gal4 UAS-GFP | 48 h ACI, 12 min hs | 16 h APF |
| 4a–d | w; UAS-optoDroncCRY2-GFP/ E-cad-tdTomato(KI); MKRS/act<y < Gal4 UAS-GFP | 48 h ACI, 12 min hs | 16 h APF |
| 4e–g | w; UAS-optoDroncCRY2-GFP/ E-cad-tdTomato(KI); UAS-p35/act<y < Gal4 UAS-GFP | 48 h ACI, 12 min hs | 16 h APF |
| 4h–j | hs-flp22; E-cad-tdTomato(KI)/Cyo; act<y < Gal4, UAS-GFP/UAS-hid-dsRNA | 48 h ACI, 12 min hs | 20 h APF |
| S5a–d | w; E-cad-GFP(KI)/UAS-α-tubulin-mCherry; pnr-Gal4/+ | – | 30 h APF |
| S5e | w; UAS-α-tubulin-mCherry/UAS-GC3AI; pnr-Gal4/+ | ||
| S5f | w; sqh-sqh-mKatex3/ UAS-optoDroncCRY2-GFP; act<y < Gal4, UAS-BFP/UAS-p35 | 48 h ACI, 12 min hs | 30 h APF |
| 5a–h | w; E-cad-tdTomato(KI)/+;pnr-Gal4/UAS-EB1-GFP | – | 16 h APF |
| 5i–k | hs-flp22; sqh-sqh-mKatex3/UAS-vhhGFP-CRY2-CIBN; act<y < Gal4, UAS-BFP/UAS-αTubulin-GFP | 48 h ACI, 12 min hs | 16 h APF |
| 5k | hs-flp22; E-cad-mKatex3(KI)/UAS-vhhGFP-CRY2-CIBN; act<y < Gal4, UAS-GFP/+ | 48 h ACI, 12 min hs | 16 h APF |
| S6 a–e | w; sqh-sqh-mKatex3/Cyo; pnr-gal4/TM6b | – | 16 h APF |
| S6f | hs-flp22; sqh-sqh-mKatex3/UAS-vhhGFP-CRY2-CIBN; act<y < Gal4, UAS-BFP/UAS-αTubulin-GFP | 48 h ACI, 12 min hs | 16 h APF |
| 6a–d | hs-flp22; tub-Gal80ts/ ubi-E-cad-GFP, UAS-RFP; UAS-Spastin/act<y < Gal4 | 96 h ACI 18 °C, 12 min hs | 16 h APF |
| 6a–d | hs-flp22; tub-Gal80ts/ ubi-E-cad-GFP, UAS-RFP; MKRS/act<y < Gal4 | 96 h ACI 18 °C, 12 min hs | 16 h APF |
| 6e–g | hs-flp22; ubi-E-cad-GFP, UAS-RFP/+; act<y < Gal4/UAS-hid-dsRNA | 48 h ACI, 12 min hs | 16 h APF |
| 6h–j | hs-flp22; E-cad-tdTomato(KI)/UAS-GC3AI; act<y < Gal4, UAS-His3-mIFP/UAS-hid-dsRNA | 48 h ACI, 12 min hs | 16 h APF |
| 6k, l | hs-flp22; E-cad-tdTomato(KI)/ UAS-optoDroncCRY2-GFP; act< y < Gal4, UAS-GFP/UAS-p35 | 48 h AIC, 12 min hs | 16 h APF |
| 6m–o | w; E-cad::3xmKate(KI)/+; pnr-Gal4/UAS-EB1-GFP | – | 16 h APF |
| S7a | hs-flp22; endocadGFP, tub-Gal80ts/+; Act<y < Gal4 UAS-nls-RFP/UAS-Spastin | 96 h ACI 18 °C, 12 min hs | 16 h APF |
| S8c, d | w; E-cad-GFP(KI)/UAS-α-tubulin-mCherry-ABC; pnr-Gal4/+ | – | 16 h APF |
| S8c, d | w; E-cad-GFP(KI)/UAS-α-tubulin-mCherry; pnr-Gal4/+ | – | 16 h APF |
ACI time after clone induction, APF after pupal formation, hs heat shock at 37 °C.