Fig. 2.

(A) Compounds 14–19 synthesized for solubility studies and photoaffinity probes 20, 21 and 22 designed to evaluate photolabeling activities (B) Coomassie stained gel of photolabeled Con A and control samples (C) Western blot analysis to detect biotinylation of Con A through photolabeling. The western blot indicates that Con A is photolabeled with the pyridinyl and pyrimidinyl photolabels 21 and 22 as effectively as the conventional phenyl derived photolabel 20. (D) Photolabeling of Con A with 21 and 22 in the presence of the native ligand mannose at different concentrations. The western blot analysis suggests that the extent of photolabeling of Con A with photoprobes 21 or 22 correlates indirectly to the concentration of competing mannose ligand.