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. 2022 May 28;117(6):1464–1478. doi: 10.1111/mmi.14921

FIGURE 6.

FIGURE 6

Inhibition of Pshp144 transcription using supernatants containing mutant SHPs. (a) Cells from the reporter strain (Pshp144:: lacZ‐wt) were incubated with the supernatants of wild type, or mutant Shp144 producing strains. The supernatants were obtained at the late exponential phase. (b) Dose‐dependent inhibition of Pshp144 transcription by using supernate containing SHP P21A. Wild type and modified supernates were serially diluted, and incubated with Pshp144:: lacZ‐wt to an OD600 (~0.6), and the Pshp144 activity was measured by β‐galactosidase assay (n = 3). The error bars represent the standard error of the mean. *p < .05, “ns” not significant compared to the wild‐type.