TABLE 1.

Transcription activation and binding capabilities of native and modified SHPs

Peptide a	Transcription activation b (Miller units/109 cells)	Binding affinities (KD c in μM)
C13 (WT)	215 ± 5	6.6 ± 0.3
S14A	105 ± 5	8.1 ± 0.9
E15A	107 ± 2.5	3.3 ± 0.1
W16A	7.5 ± 2.5	50.0 ± 3.6
V17A	6 ± 1	9.7 ± 0.6
I18A	7.5 ± 1.5	>100
V19A	215 ± 5	4.0 ± 0.8
I20A	8.5 ± 0.5	23.54
P21A	7.5 ± 2.5	1.7 ± 0.1
F22A	90 ± 10	7.1 ± 0.4
L23A	95 ± 5	4.3 ± 0.2
T24A	175 ± 5	3.3 ± 0.1
N25A	185 ± 5	3.3 ± 0.2
L26A	182 ± 2.5	3.4 ± 0.3

^a WT C13 or modified peptides were used. The numbers show the position of alanine replacement within the full‐length Shp144.

^b Mean ± SEM of three independent experiments.

^c Dissociation constant between the Rgg144 and its ligand SHP144.