FIG. 2.

Northern analysis of expression of sutA, sutB, parA, pcbC, and actA in mycelium of the P. chrysogenum strains Wisconsin 54-1255 (Low), Panlabs P2 (Med), and GB8 (High) after growth under (A) sulfur starvation (−S) and sulfur-sufficient (+S) conditions and (B) under penicillin-producing (+SP) and glucose-repressed (+SnP) conditions. Total RNAs (A, 10 μg for Wisconsin 54-1255 and Panlabs P2 and 3 μg for GB8; B, 10 μg for all strains) were separated by agarose-formaldehyde gel electrophoresis, transferred to nylon membranes (Bio-Rad Laboratories, Hercules, Calif.), and hybridized with ³²P-labeled probes, as previously described (21). The data shown are for a single experiment, but similar results were obtained in two other independent experiments.