Fig. 1.

ISM1 deficiency leads to increased leukocyte infiltration in the lung under ambient condition. a Increased immune cell infiltration in the lungs of Ism1^−/− mice at 2 months via H&E staining. b Increased macrophages and neutrophils in the lungs of Ism1^−/− mice at 2 months detected by IHC staining for CD68 and NIMP-R14, respectively. c–e Differential immune cell count showed increased total leukocytes, alveolar macrophages, and neutrophils in Ism1^−/− lungs. f Analysis of the peripheral blood of Ism1^−/− mice showed increased total white blood cells, neutrophils and lymphocytes at 2 months. K/µl count per µl, WBC white blood cell, NE neutrophil, LY lymphocyte, MO monocyte, EO eosinophil, BA basophil. *represents p < 0.05; **represents p < 0.01. For a-e, n = 4 mice per group; for f, n = 7 mice per group