Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 25;28:72. doi: 10.1186/s10020-022-00500-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Increase of AMs in Ism1^−/− mouse under LPS is mainly due to increase of Mo-AMs. BALF of 8–9 weeks old WT and Ism1^−/− mice was collected at day 3 and day 5 after intratracheal administration of 2 mg/kg LPS (n = 4–5). After BALF collection, cells were isolated, erythrocytes were lysed, and stained with Viobility fixable dye for live/dead cells, various marker antibodies and Annexin-V and analyzed by flow cytometry. Total cells from BALF were presented in panel a-d. The flow cytometry gating strategy is shown in Additional file 1: Fig. S5. a Quantification of the amount of TR-AM and Mo-AM. b Percentage of early apoptotic Mo-AM as identified as Annexin-V⁺ in Mo-AM population. c Percentage of early apoptotic TR-AM as identified as Annexin-V⁺ cells in TR-AM population. d Percentage of early apoptotic neutrophils as identified as Annexin-V⁺ cells in neutrophil population. e Representative histograms showing the percentage of Annexin-V⁺ cells in Mo-AM, RT-AM, and neutrophil population on day 5 post LPS. Statistical analysis was conducted by Student's t-test. “*” represents P < 0.05; “***” represents P < 0.001