Fig. 6.

ISM1 deficiency increases proliferation of alveolar epithelial type 2 cells (AT2) and elevates profibrotic TGF-β in mouse lung. a Representative images of SP-C and PCNA double IF stained lung sections of Ism1^−/− and WT mice at day 9 post LPS challenge. b Quantification of the percentage of SP-C⁺PCNA⁺ cells in the lung of Ism1^−/− and WT mice at day 9 post LPS challenge. Increased number of proliferating AT2 cells in the lungs of Ism1^−/− mice were shown. *** represents p < 0.001. n = 3 mice per group, 2 sections per lung, 5 microscopic fields per section were analyzed. c Representative images of IF stained TGF-β lung sections of Ism1^−/− and WT mice at day 9 post LPS challenge. n = 3 animals per group, 2 sections per lung, 5 microscopic fields per section were analyzed. d, e TGF-β protein level in the lung of Ism1^−/− and WT mice at day 9 post LPS challenge by Western blot of whole lung lysate d and quantification (e). ** represents p < 0.01. n = 3 mice per group, 1 total lung lysate per mouse