Figure 4. Differential regulation of ILC1 populations across tissues by T-bet, Eomes and TGF-β.

A. Representative (left) flow cytometric analysis of granzyme C (GzmC) and CD49a expression among NK1.1⁺CD3⁻ cells in the liver (upper) and salivary gland (lower) of wild-type (WT), GzmC^iCreTbx21^fl/fl (Gzmc^ΔTbx21) (yellow), GzmC^iCreEomes^fl/fl (Gzmc^ΔEomes) (green), and GzmC^iCreTbx21^fl/flEomes^fl/fl (Gzmc^{ΔTbx21Δeomes}) (blue) mice. (Right) abundance of GzmC⁺NK1.1⁺CD3⁻ (GzmC⁺) and CD49a⁺NK1.1⁺CD3⁻ (ILC1) cells out of total CD45⁺ cells in liver and salivary gland. B. Representative (left) and quantification (right) of flow cytometric analysis of group 1 innate lymphocytes in liver (upper) and salivary gland (lower) of WT (white bar) and GzmC^iCreTgfbr2^fl/fl (Gzmc^ΔTgfbr2) (gray bar) mice, quantifying CD49a⁺ cells among NK1.1⁺CD3⁻ cells and CD103⁺ and GzmC⁺ cells among CD49a⁺NK1.1⁺CD3⁻ cells in each organ. Each dot represents one mouse (n = 4–10 mice per group). All data are combined from three or more independent experiments and shown as mean +/− SEM (unpaired student’s t-test for two groups, one-way ANOVA with Tukey’s multiple comparisons test with more than two groups, “ns” = not significant, * = p<0.05, ** = p<0.01, *** = p<0.001, **** = p<0.0001).