Figure 4.
CID treatment of mono- and bialleic iPSCs led to selection of rare CID-resistant cell subclones from monoallelic, but not biallelic, iCASP9 iPSC clones
Frequencies of rare CID-resistant cell subclones/CID-treated cells in individual experiment are shown. Monoallelic and biallelic iPSCs were treated with CID (concentrations from 0.5 to 10 nM AP1903/AP20187), seeded onto irradiated feeder cells, and cultivated for 3 weeks to promote the propagation of potentially surviving cells. Survival of apparently CID-resistant extremely rare cell clones was observed in several independent experiments (1 × 105 to 1 × 108 cells/experiment) with overall frequencies of 3.6 × 10-8 (hCBiPS2) or 2.5 × 10-8 (Phoenix). We never observed surviving clones in biallelic transgenic iPSCs despite the high number of treated cells. Data are presented as mean ± SD (n = 21–27). D'Agostino-Pearson omnibus normality test. Kruskal-Wallis test (p < 0.01).