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. 2022 Jun 26;79(7):384. doi: 10.1007/s00018-022-04407-x

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© The Author(s) 2022

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Fig. 3 — Confirmation of syncytialisation by staining of cell membranes. A Fluorescent image of PKH67 staining of cultures of cytotrophoblasts from a placentae of 39 weeks of gestation (isolated using the protocols described in [110]), in which spontaneous syncytiotrophoblast differentiation has occurred over the 30 day culture period. Nuclei are counterstained with Hoechst 33342. Multinuclear clusters within a single membrane (demonstrating syncytiotrophoblast) are indicated with white arrows. Scale bar = 100 μm