Figure 6.

TUFT1 suppressed ZYX expression and inhibited its recruitment to focal adhesion. (a) Co-IP of TUFT1-Flag with an anti-Flag antibody (upper panel), or with an anti-ZYX antibody (down panel), followed by western blot analysis in Huh7 cells. (b) Co-immunofluorescence analysis of ZYX and TUFT1 in HCC cells, with colocalization at focal adhesions enlarged with a white rectangle; scale bar: 10 μm. (c) Western blot analysis of ZYX in TUFT1-overexpressing or knockdown cells. (d) Co-immunofluorescence analysis of ZYX and paxillin in TUFT1-overexpressing or knockdown HCC cells. Colocalization in yellow at cell-substratum adhesion showed mature focal adhesions. Scale bar: 5 μm.