Digestion mix from Miltenyi Whole Skin Dissociation Kit (see key resources table)

Reagent	Aliquots preparation	Amount per reaction
Enzyme P	25 μL aliquots	12.5 μL
Enzyme D	Dissolved in Buffer L, 50 μL aliquots	50 μL
Enzyme A	Dissolved in Buffer L, 10 μL aliquots	2.5 μL
Buffer L	30 mL, do not aliquot	435 μL
Total	50 reactions per Kit	500 μL

Store Buffer L at 4°C and enzymes at −20°C until Kit expiration date, avoid freeze/thaw cycles.