REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Human BD Fc Block	BD Biosciences	Cat#: 564219
κPE/CF 594 anti-human CD45, 1:100	BD Biosciences	Cat#: 562279
PE anti-human HLA-ABC, 1:200	BioLegend	Cat#: 311405
BV421 anti-human CD3, 1:200	BioLegend	Cat#: 300434
PE-Cy7 anti-human CD90, 1:100	BioLegend	Cat#: 328124
AF488 anti-human CD34, 1:50	BD	Cat#: 345801
7-AAD Viability Staining Solution, 1:500	eBioscience	Cat#: 00-6993-50
TotalSeq-C0251 anti-human Hashtag 1 Antibody	BioLegend	Cat#: 394661
TotalSeq-C0252 anti-human Hashtag 2 Antibody	BioLegend	Cat#: 394663
Chemicals, peptides, and recombinant proteins
Fetal bovine serum (FBS)	Gibco	Cat#: 10500-064
EDTA	Thermo Fisher Scientific	Cat#: 15575-020
Bovine serum albumin (BSA)	Sigma	Cat#: A8412-100mL
PBS	Gibco	Cat#: 10010-023
RPMI 1640 Medium	Thermo Fisher Scientific	Cat#: 11875093
Dimethyl sulfoxide (DMSO)	Sigma	Cat#: D2650
Critical commercial assays
Whole Skin Dissociation Kit, human	Miltenyi Biotec	Cat#: 130-101-540
Software and algorithms
FlowJo	Tree Star	https://www.flowjo.com/solutions/flowjo
Seurat v4.0.1	Stuart et al. (2019)	https://github.com/satijalab/seurat
CITE-seq software	Stoeckius et al., 2017	https://cite-seq.com/
Other
gentleMACS™ C Tubes	Miltenyi Biotec	Cat#: 130-093-237
gentleMACS™ Octo Dissociator	Miltenyi Biotec	Cat#: 130-095-937
70 micron cell strainer	Falcon	Cat#: 352350
Scalpel No.11	Medi-Safe Surgicals	Cat#: BYDMSS11