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. 2022 Jun 13;13:903565. doi: 10.3389/fneur.2022.903565

Figure 3.

Figure 3

Metformin, rapamycin, or NMN pretreatment improves axonal and oligodendrocyte damage induced after BCCAO. (A) Representative images of dephosphorylated neurofilament protein (SMI32, red) and MBP (green) in striatum 32 days after BCCAO in rats. (B) Quantification of the ratio of SMI32 to MBP fluorescence intensity in the striatum, illustrated as fold-change compared with the sham average value. n = 6 per group. The p-values were assessed by one-way ANOVA with a Tukey's test. (C) Western blot analysis of MBP protein from the striatum in sham and BCCAO rats after treatment. (D) Quantitative analysis of MBP protein from the striatum in each group. MBP protein was normalized to the β-actin level of the same sample (n = 4 per group). The p-values were assessed by one-way ANOVA with a Tukey's test. All data were shown as mean ± SEM. Met, metformin; Rapa, rapamycin; NMN, nicotinamide mononucleotide; Veh, vehicle; 2VO, two-vessel occlusion.