Figure 5.

Metformin, rapamycin, or NMN pretreatment alleviates microglial phagocytosis of myelin in the striatum after BCCAO. (A) Photomicrographs of triple immunofluorescent staining for Iba1 (red), CD68 (green), and MBP (blue). White arrows indicate Iba1⁺ microglia. Yellow arrows indicate Iba1⁺CD68⁺ microglia. Green arrows indicate Iba1⁺CD68⁺ MBP⁺ microglia. (B) Quantification of CD68 (green), Iba1 (red), and MBP (blue)-positive cells in the striatum of sham-operated and BCCAO rats pretreated with vehicle (Veh), metformin (Met), rapamycin (Rapa), or NMN (NMN) for 2 weeks before surgery. The p-values were assessed by the Kruskal–Wallis test with a Dunn post-test. All data were shown as mean ± SEM. N = 6 per group. 2VO, two-vessel occlusion.