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. 2022 May 4;298(7):102010. doi: 10.1016/j.jbc.2022.102010

Figure 4.

Figure 4

FSTL1 activates β-catenin and fibroblasts.A, FSTL1 protein elevated active β-catenin levels induced by Wnt3a proteins in NRK-49F cells. FSTL1 protein (500 ng/ml) and Wnt3a (15 ng/ml) were preincubated overnight before they were added to serum-starved NRK-49F cells for 3 h. Cell lysates were used for Western blotting for active β-catenin levels (upper panel). Levels of active β-catenin relative to GAPDH are presented (lower panel). B–D, transfected FSTL1-HA promoted β-catenin levels and fibroblast activation induced by transfected Wnt3a in NRK-49F cells. Cells were transfected with Wnt3a plasmid (50 ng/ml) in the presence or the absence of FSTL1-HA plasmid (200 ng/ml). Cells were serum starved for 24 h before cells were harvested for Western blotting for p-LRP6, LRP6, active β-catenin, β-catenin, fibronectin (Fn-1), collagens (Col 1A1 and Col 1A2), FSTL1, and Wnt3a (B). Levels of p-LRP6 relative to LRP6, active β-catenin relative to β-actin, and β-catenin relative to β-actin were presented (C). Levels of Fn-1, Col 1A1, and Col 1A2 relative to β-actin were also presented (D). FSTL1, follistatin-like 1; HA, hemagglutinin; NRK-49F, normal rat kidney 49 fibroblast cell.