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. 2022 Jun 13;10:874323. doi: 10.3389/fcell.2022.874323

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Copyright © 2022 Robinson, Ali, Diaz-Hernandez and Lopez-Gonzalez.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of the expression of telomere maintenance genes during the differentiation of iPSCs to MNs from controls and C9ORF72 repeat expansion carriers. qPCR analysis to compare expression levels (A) TRF1, (B) TRF2 and (C) POT1 transcripts in NEP and levels of (D) TRF1, (E) TRF2 and (F) POT1 in MNP from 3 control subjects and 3 C9ORF72 carriers, from 3 independent differentiations. Western blot analyses to compare protein levels of (G) TRF2 and (H) POT1 in NEP and protein levels of (I) TRF2 and (J) POT1 in MNP, from 3 controls and 3 C9ORF72 iPSC lines, Two-tailed t-test with Welch’s correction was applied. ns, not significant.