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. 2022 Jun 21;20:434–448. doi: 10.1016/j.bioactmat.2022.06.006

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© 2022 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 4 — Corneal epithelial cells and corneal stromal cells cultured on the HAMA, pDCSM-G, C3H1 hydrogels, and TCP. (A) Live/Dead staining of corneal epithelial cells cultured on 1% HAMA, 3% pDCSM-G, C3H1 hydrogel, and TCP for 1, 4, and 7 days, respectively. Scale bars = 200 μm. (B) Quantification of the corneal epithelial cells viability on hydrogels and TCP characterized by Live/Dead assay. (C) CCK-8 assay of the cultured corneal epithelial cells after 1, 4, and 7 days. (D) Live/Dead staining of corneal stromal cells cultured on 1% HAMA, 3% pDCSM-G, C3H1 hydrogel, and TCP for 1, 4, and 7 days. Scale bars = 200 μm. (E) Quantification of the corneal stromal cells viability on hydrogels and TCP characterized by Live/Dead assay. (F) CCK-8 assay of the cultured corneal stroma cells after 1, 4, and 7 days. (G) qRT-PCR analysis of the relative mRNA expression levels of Collagen 1A1, Collagen 3A1, ACTA2, TGF-β1, lumican, and keratocan in the rabbit corneal stromal cells after culturing on the C3H1 hydrogel and TCP for four days. (*P＜0.05, **P＜0.01, ***P＜0.001, ****P＜0.0001).