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. 2022 Jun 13;13:843732. doi: 10.3389/fphar.2022.843732

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Copyright © 2022 Martínez-Salgado, Sánchez-Juanes, López-Hernández and Muñoz-Félix.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Impaired peritubular capillaries rarefaction in Alk1 ^+/− mice. (A) CD31 immunostaining in SO and O kidneys from Alk1 ^+/+ and Alk1 ^+/− mice and blood vessel density analysis, represented as CD31 ⁺ vessels per field. (B) Endomucin immunofluorescence staining in SO and O kidneys from Alk1 ^+/+ and Alk1 ^+/− mice and blood vessel density quantification from endomucin staining, represented as microvessels per field (upper graph) and endomucin+ cells per field (lower graph) in SO and O kidneys from Alk1 ^+/+ and Alk1 ^+/− mice. *p < 0.05; ***p < 0.001; N.S. Not statistically significant (Two-way ANOVA). Squares in (A) indicate the zoomed areas. Scale bar = 200 microns in both panels. Blood vessels from the glomeruli in (B) (highlighted as cropped áreas) were not counted.