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. 2022 Jun 13;13:843732. doi: 10.3389/fphar.2022.843732

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Copyright © 2022 Martínez-Salgado, Sánchez-Juanes, López-Hernández and Muñoz-Félix.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ALK1 and VEGF protein expression after UUO. (A) Western blot analysis of ALK1 protein in SO, NO and O kidneys from Alk1 ^+/+ and Alk1 ^+/− mice (N = 5), and quantification of the corresponding densitometry analysis. Bars represent the ratio between the proteins and GAPDH, used as loading control. (B) VEGF immunohistochemistry representative pictures and quantification of VEGF levels (using Fiji software) in SO, NO and O kidneys from Alk1 ^+/+ and Alk1 ^+/− mice. *p < 0.05; **p < 0.01 N.S. Non statistically significant (Two-way ANOVA). Scale bar = 150 microns.