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. 2022 Jun 13;9:928235. doi: 10.3389/fvets.2022.928235

Table 2.

Hematological and biochemical parameters of 5-week-old broilers fed different levels of dietary algae.

Treatment (g/kg)
Parameter Control Sargassum Sargassum Spirulina Spirulina Gracilaria Gracilaria SEM P-value
sp. (1%) sp. (2%) sp. (5%) sp. (7.5%) sp. (0.5%) sp. (1%)
WBC (K/ul) 99.35a 90.05b 94.45b 85.45c 89.30b 90.56b 94.59b 2.52 0.012
Heterophils (%) 10.05a 11.20a 3.05b 5.48b 6.22b 5.23b 6.01b 2.13 0.017
Lymphocytes (%) 0.85b 1.17b 47.90a 31.30a 25.10a 36.23a 38.63a 4.34 0.002
Monocytes (%) 21.50b 47.50a 25.20b 22.45b 23.20b 20.25b 21.32b 0.66 <0.001
Eosinophils (%) 0.010ab 0.00b 0.006b 0.012ab 0.022a 0.00b 0.005b 0.003 0.016
Basophils (%) 38.05b 39.15b 23.85b 39.25b 54.45a 48.54a 50.10a 2.71 0.005
RBC (M/ul) 2.71 2.71 2.68 3.20 2.66 3.11 2.9 0.36 0.802
HGB (g/dl) 13.15 13.00 12.60 16.00 13.45 14.12 13.99 1.93 0.751
HCT% 31.20 30.05 31.80 38.00 31.30 32.10 31.78 5.32 0.834
MCV (fL) 115.00 111.50 116.00 118.00 117.00 115.98 117.10 5.43 0.913
MCH (pg) 48.40 47.55 46.45 49.80 50.50 50.12 49.51 1.70 0.510
MCHC (g/dl) 42.05 42,.80 40.10 42.10 42.90 42.05 42.00 0.94 0.346
RDW% 14.75 15.20 14.15 13.45 13.25 14.20 13.99 0.80 0.449
Thrombocytes/PLT (K/ul) 3.2 2.63 1.75 1.84 0.57 1.50 0.98 0.76 0.286

Means within rows are significantly different at p ≤ 0.05, n = 5.

SEM, Standard error of the mean, calculated by one-way analysis of variance (ANOVA) and the general linear model procedure of Minitab; WBC, white blood cells; RBC, red blood cells; HGB, hemoglobin; HCT, hematocrit; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; RDW, red cell distribution width; PLT thrombocytes.

a, bMeans in a column with no common superscripts are significantly different (p ≤ 0.05).