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. 2022 Jun 13;13:892251. doi: 10.3389/fimmu.2022.892251

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Copyright © 2022 Topping, Romero-Castillo, Urbonaviciute, Bolinsson, Clanchy, Holmdahl, Bäckström and Williams

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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High reproducibility of delayed-type hypersensitivity (DTH) response induced by immunization using the POWER-Kit™ method. (A) Comparison of two independent DTH experiments using the POWER-Kit™ method. To induce DTH, HLA-DRB1*0401.hIi.cia9i mice were immunized with 100 µg of bCol2 in CFA on day 0 i.d. On day 8, 10μg of bCol2 in PBS was injected intradermally into the left ear and PBS to the right ear. The ear thickness was measured at 24, 48, and 72h. (B) Comparison of two independent delayed-type hypersensitivity (DTH) experiments performed using the manually prepared emulsions or using the POWER-Kit™ device.