Table 2.

The sequence of 16s rRNA qRT-PCR primers used to quantify intestinal bacteria.

Target	Primer sequence (5′-3′)	Amplicon size, bp	Reference
Clostridium	F: ATGCAAGTCGAGCGAKG	105	(27)
perfringens	R: TATGCGGTATTAATCTYCCTTT
Lactobacillus	F: AGCAGTAGGGAATCTTCCA	341	(25)
subgroup	R: CACCGCTACACATGGAG
Escherichia	F: GTTAATACCTTTGCTCATTGA	340	(24)
subgroup	R: ACCAGGGTATCTAATCCTGT