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. 2022 Jun 13;9:815467. doi: 10.3389/fmed.2022.815467

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Copyright © 2022 Cheng, Zhou, He, Liu, Huang, Niu, Liu, Gao, Lu, Song, Li, Xiao, Wang and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Metabolomics analysis of ALD patients with/without ascites. (A–D) PCA and orthogonal partial least squares discrimination analysis (OPLS-DA) plots with all metabolic variables in NA, MA, and LA patients under positive and negative models of mass spectrometry, respectively. (E) Metabolites with significant differences in MA and LA, in comparison to NA. There are 553 metabolites uniquely associated with ascites, with 136 metabolites annotated in the Human Metabolome Database. (F) PCA plot of MA + LA vs. NA with 136 annotated metabolites related to ascites. The QC samples are clustered together in the PCA plots, indicating stability and technical reproducibility.