FIGURE 6.

Scn9a^R185H mutant mice show spontaneous ongoing pain. Ongoing pain was assessed in a conditioned place preference test. After pre-test/habituation for chamber preference on days 1 to 3, conditioning was made on day 4 by intrathecal treatment with either saline solution in the morning or clonidine solution in the afternoon with a restriction in one chamber. On day 5, the animals were free to explore the chambers and the time spent in each chamber was recorded for 30 min. (A) Females. (B) Males. The time spent in each chamber before conditioning (pre-test) and after conditioning with clonidine or saline (test) are shown. Homozygous mice of both sexes spent more time in clonidine-paired chamber while wt mice spent on average equal time in saline-paired and clonidine-paired chambers. Dots represent individual data points. females: wt, n = 18; Homo, n = 18; males: wt, n = 10; Homo, n = 10. Two-way ANOVA for treatment and trial (pretest and test) on each genotype and sex group, Sidak’s multiple comparison test. P = values for comparing pre-test and test values are shown. Refer to Supplementary Table 12 for statistics.