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. 2022 May 31;135(11):jcs259617. doi: 10.1242/jcs.259617

Fig. 1.

Fig. 1.

VEGF-A affects DAD2 receptor expression in endothelial cells. Knockdown of VEGF-A in HT29 cells was confirmed by (A) real-time qPCR and (B) ELISA. Data are expressed as the mean±s.e.m. (n=3). *P<0.05 (two-sample two-tailed t-test). (C) Representative images of colocalization (arrows) of CD31 and DAD2 receptors [i.e. expression of DAD2 receptors on the tumor endothelial cells (TECs)]. TECs (green) of HT29+VEGF-A+/+ tumors showed significantly higher expressions of DAD2 receptors (red) compared to TECs of HT29+VEGF-A−/− tumors and endothelial cells (ECs) of normal colon tissues (n=10 for each group). Scale bars: 80 µm. Right, ten fields of view were randomly chosen and analyzed. Data are expressed as the mean±s.e.m. *P<0.05 for HT29+VEGF-A+/+ versus normal; +P<0.05 for HT29+VEGF-A−/− versus HT29+VEGF-A+/+ (one-way ANOVA with Bonferroni step down adjustment). (D) Representative images of colocalization (arrows) of CD31/dopamine D2 receptors indicate high expressions of DAD2 receptors on TECs of VEGF-A secreting well-differentiated human colon adenocarcinoma tissues compared to the ECs of normal colon tissues (n=45 for each group). Scale bars: 80 µm. Right, four fields of view per section were randomly chosen and analyzed. Data are expressed as the mean±s.e.m. *P<0.05 for tumor versus normal (two-sample two-tailed t-test).