Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Jun 6;135(11):jcs259465. doi: 10.1242/jcs.259465

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

PMC Copyright notice

Fig. 7. — Integrin β5 in clathrin lattices mediates adhesion to vitronectin ∼4 h after cell seeding. (A,B) SW620 cells were seeded on vitronectin-coated coverslips and fixed at the indicated time points. Representative immunofluorescence images showing integrin β5 (green in merge), vinculin (A) or clathrin (B) (red in merge), actin in blue, and the cell nuclei in cyan. Scale bars: 20 μm. Representative images are shown of two independent experiments performed in duplicate. (C,D) Adhesion assay performed 1.5 h (C) and 4 h (D) after seeding SW620 wild-type (WT) and β5 knockout cells on vitronectin (VN) (three biological replicates; each experiment in triplicate; bars show mean±s.d). **P<0.01 (two-sided unpaired Student's t-test).