FIGURE 3.

Silencing of the HBV S gene by CBE‐mediated base editing. (A) CAG to TAG editing was obtained in PLC/PRF/5‐CBE cells transduced with LV‐gRNA_S. Representative sequencing chromatogram of the amplicon obtained from PLC/PRF/5‐CBE cells transduced with either an empty lentiviral vector (LV‐empty) or lentiviral vectors expressing nonspecific sgRNA (LV‐NS‐gRNA) or gRNA_S (LV‐gRNA_S). Red arrows indicate the edited nucleotides. (B) The output plots of Sanger sequencing traces by EditR. Percentage of T editing at each position was depicted. C‐to‐T editing was only observed in the gRNA_S group. (C) The percentage of TAG in the 30th codon of HBV S gene was shown for treatment with LV‐gRNA_S