Fig. 3.

Alignment of the consensus sequences of Populus NRAMP3.1 and NRAMP3.2. The consensus sequences were determined from the NRAMP alignments shown in supplementary figure S3, Supplementary Material online. Asterisk indicates less than 90% of conservation within Populus NRAMP3.1 or NRAMP3.2 cluster. Identical, similar, and different residues between the two consensus sequences are indicated in black, blue, and red, respectively. Frames indicate residues under purifying selection and green arrows indicate positive selection in either Populus NRAMP3.1 or NRAMP3.2 cluster according to the FEL method (P < 0.05). Note that NRAMP3.1 sequences contain an insertion of residue at position 4, leading to a gap (–) in NRAMP3.2 sequences.