Fig. 4.

PotriNRAMP3.1 and PotriNRAMP3.2 encode functional Mn transporters. Functional complementation of smf1 (A) and smf2 (B) yeast mutants. Yeast cells expressing GUS (negative control), AtNRAMP1 (positive control), AtNRAMP2 (positive control), PotriNRAMP3.1, or PotriNRAMP3.2 were grown overnight. The cultures were diluted to ODs of 1–10⁻³ and spotted on synthetic dextrose -ura plates. Transformed smf1 (A) strains were grown on medium supplemented with 5 mM EGTA and 100 µM MnSO₄ (+Mn) or with 5 mM EGTA without MnSO₄ (–Mn). Transformed smf2 (B) strains were grown in medium supplemented with 10 mM EGTA and 100 µM MnSO₄ (+Mn) or with 10 mM EGTA without MnSO4 (–Mn). The plates were incubated at 30°C for 5 days (smf1) or 2 days (smf2) before photography. White lines indicate cropping.