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. 2022 Jun 14;39(6):msac129. doi: 10.1093/molbev/msac129

Fig. 5.

Fig. 5.

PotriNRAMP3.2 but not PotriNRAMP3.1 expression complements the Arabidopsis nramp3nramp4 double mutant growth defects under iron starvation. (A) Representative pictures of nramp3nramp4 pUb10:PotriNRAMP3.1 and pUb10:PotriNRAMP3.2 T3 Arabidopsis lines together with wild-type (Col-0) as positive control and nramp3nramp4 as negative control grown vertically for 8 days in ABIS supplemented with 50 μM FeHBED (control, bottom panel) or without iron (–Fe, top panel). (B) Quantification of main root lengths of wild-type (Col-0), nramp3nramp4 and 3 independent nramp3nramp4 pUb10:PotriNRAMP3.1 and pUb10:PotriNRAMP3.2 T3 lines. Values represent mean of 10–12 roots and bars represent SD. Different letters reflect significant differences according to a Kruskal–Wallis test followed by Dunn’s test for multiple comparison (P < 0.01). No significant differences among genotypes were detected in the presence of Fe, left panel in (B).