Figure 3.

PK/PD study with CCT373566 at 50 mg/kg po. Tumor xenografts were prepared by subcutaneous injection of 1.5 × 10⁷ OCI-Ly1 cells in female SCID mice, with dosing of the compound commencing 20 days after injection, to mice with xenografts between 0.5 and 0.8 cm³, as described in more detail in the Supporting Information. Sampling took place at 12, 16, and 24 h after dosing. All experiments were carried out according to the U.K. guidelines for animal experimentation. BCL6 levels in the tumor were quantified using capillary electrophoresis and normalized to a GAPDH loading control and are shown as black (vehicle-treated) or white (compound-treated) bars. Free compound levels at 12–24 h are shown (red dots); the dotted line indicates free DC₅₀.