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. 2000 Oct;66(10):4585–4588. doi: 10.1128/aem.66.10.4585-4588.2000

FIG. 3.

FIG. 3

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of concentrated (10×) supernatants of suspensions of a Burkholderia sp. The gel was stained using a silver-staining procedure for LPS. Lanes: 1, buffer; 2, 5 μg of BSA; 3, 4485.6 μg (890 μM) of rhamnolipid; 4, supernatant of the Burkholderia sp. treated only with MSM; 5, supernatant of the Burkholderia sp. treated with 8.9 μM rhamnolipid; 6, supernatant of the Burkholderia sp. treated with 89 μM rhamnolipid; 7, supernatant of the Burkholderia sp. treated with 890 μM rhamnolipid; 8, 1 μg of P. aeruginosa serotype 10 LPS; 9, 10 μg of P. aeruginosa serotype 10 LPS. The gel was imaged and the band density (integrated density value) was determined using the SpotDenso function of AlphaImager (Alpha Innotech, San Leandro, Calif.).