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. 2022 Jun 3;13:904536. doi: 10.3389/fgene.2022.904536

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Copyright © 2022 Yao, Liu, Yang, Li, Li, Zhang, Yang, Zhang, Cui and Feng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The CSC subgroup was identified and verified by evaluation of stemness (A) t-SNE plot of malignant epithelial cells isolated across all specimens, colored and labeled by cluster. The cells of clusters-7 in the green box have higher stemness (B) t-SNE plot showing the stemness score for each cell. The color of the dots represents the stemness, and the darker the color, the higher the stemness. The cells in the green box have the highest stemness (C) Box plot showing the stemness score for each cluster. Cluster-7 has the highest stemness (D) ssGSEA score of CSCs-related gene set in each seurat_clusters. Top: Represent the gene set of stem genes upregulation. Bottom: Represent the gene set of stem genes downregulation.