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. 2022 Jun 27;12(6):e741. doi: 10.1002/ctm2.741

FIGURE 7.

FIGURE 7

HDAC2 is essential for the TRIB2‐mediated CSC properties and radio resistance of ESCC cells (A) Representative images(left) and statistical quantification (right) of sphere formation in the indicated ESCC cells treated with or without the HDAC2 inhibitor (SCA,2µM). Scale bars: 100µm. (B–C) Representative images and statistical quantification of the flow cytometric analysis determining the ALDH activity and proportion of the CD90‐positive and CD271‐positive subpopulations in the indicated ESCC cells treated with or without HDAC2inhibitor (SCA, 2µM). (D) Western blotting analysis of CSC markers in the indicated ESCC cells treated with or without the HDAC2 inhibitor(SCA, 2µM). (E) Representative images (left) and statistical quantification (right) of sphere formation by the indicated ESCC cells treated with or without the HDAC2 inhibitor (SCA, 2µM). Scale bars: 100µm. (F‐G) Representative images and statistical quantification of the results of flow cytometric analyses to determine ALDH activity and the relative proportions of CD90+and CD271+cells in the indicated ESCC