Figure 4.

Spurious variants are much more abundant in samples treated with fragmentase. (A) Design of the experiment testing different fragmentation and ER methods as well as DNA input source. Libraries were made from NA12814 and Hs27 DNA using either KAPA HyperPlus with ER mix 1 or 2 or KAPA HyperPrep with ER mix 1 (see the ‘Materials and Methods’ section). They were captured on the same probes as in Figure 2 and sequenced. (B) The variant incidence (excluding the expected variants) for the six resulting samples at single-strand and duplex consensus levels.