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. 2022 Jun 27;21:128. doi: 10.1186/s12934-022-01856-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Positive correlation between the pI value and the secretion level. A Schematic diagram of the XTHHly system. POI: Protein of interest; TEV cs: TEV enzyme cleavage site; Hly218: the last 218 amino acids of hemolysin A. B–D SDS-PAGE analysis of culture samples (n = 2). W: Whole-cell lysate; S: Supernatant. The native and mutated tag fusion proteins are indicated above the image. The arrows indicate the tag fusion proteins. C–E The relationship between the pI value and the secretion level. The secretion levels of mutated tag fusion proteins were estimated by Image J scanning. All SDS-PAGE was conducted under non-reducing conditions