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. 2022 Jun 27;20:303. doi: 10.1186/s12951-022-01505-7

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — In vivo administration of arctiin mitigated DMM-induced articular cartilage abrasion in seven-week-old C57BL/6J male mice. A Representative images of articular cartilage stained by hematoxylin and eosin (H&E). B Representative images of articular cartilage stained by safranin O and fast green (S.O.). C Representative images of articular cartilage stained by collagen type II (COLII). D Quantification of the ratio of hyaline cartilage (HC) versus calcified cartilage (CC) (n = 6). E Quantification of the Osteoarthritis Research Society International (OARSI) score (n = 6). F Quantification of the percentage of COLII-positive cells in the articular cartilage (n = 6). Values represent the mean ± SD. Statistically significant differences are indicated by * where p < 0.05, ** where p < 0.01, *** where p < 0.001 and **** where p < 0.0001 between the indicated groups