Fig. 5.

mbTBI affects BBB integrity in male rats but not in female rats. SMI-71 immunofluorescence to assess capillary barrier integrity. A Representative SMI-71 immunofluorescence micrograph from Sham and mbTBI rat brain at 24 h post-mbTBI. B 20X magnification of vascular integrity in amygdala region. C, D Vascular density quantification using ImageJ in whole brain section and amygdala region of sham and mbTBI from male and female rats 24 h post-mbTBI (n = 3–6/group). E, F Vascular density quantification using ImageJ in whole brain section and amygdala region of sham and mbTBI from male and female rats 7 d post-mbTBI (n = 3–4/group) Data represented as Mean ± SEM. P ≤ 0.05 * by Two-way ANOVA with Bonferroni post-hoc C, D, E, F